Journal:

Article Title: Acute induction of human IL-8 production by intestinal epithelium triggers neutrophil infiltration without mucosal injury

doi: 10.1136/gut.2004.061168

Figure Lengend Snippet: Neutrophil infiltration in caecum of DOX-induced FABP-rtTA × TRE2IL-8 mice is localised to discrete patches. (A) Biotin-conjugated antibody to Gr-1 followed by streptavidin-phycoerythrin was used to localise neutrophils (red) in frozen sections of FABP-rtTA × TRE2IL-8 mice induced with DOX for five days (50× magnification) and counterstained with YoPro-3 (pseudocoloured blue). Areas of intense neutrophil infiltration are separated by groups of crypts in which no neutrophils are detected. A similar patchy pattern of neutrophil infiltration was observed in sections from four other double transgenic mice stained with the same antibodies. (B) High power (600×) image of a crypt demonstrates Gr-1+ neutrophils that have migrated into the paracellular space between epithelial cells. (C) (D) Consecutive sections of caecum from five day induced double transgenic mice were stained for Gr-1 (C) and IL-8 (D) showing that Gr-1+ cells are present in the same patches where IL-8 is expressed.

Article Snippet: Neutrophils were detected in acetone fixed frozen sections using biotinylated anti-Gr-1 (BDPharMingen) followed by phycoerythrin conjugated streptavidin (Immunotech, Portland, ME, USA).

Techniques: Transgenic Assay, Staining